The expression of CYP1A,vitellogenin and zona radiata proteins in Atlantic salmon (Salmo salar) after oral dosing with two commercial PBDE flame retardant mixtures: absence of short-term responses     

Boon JP  van Zanden JJ  Lewis WE  Zegers BN  Goksøyr A  Arukwe A 《Marine environmental research》2002,54(3-5):719-724

The short-term effects of the commercial PBDE flame retardant mixtures Penta-BDE and cta-BDE on the expression of cytochrome P450 1A (CYP1A), vitellogenin (Vtg) and zona radiata proteins (Zrp) were investigated in juvenile salmon (Salmo salar). For this purpose, groups of fish were dosed twice (oral intake at days I and 4) with 10 and 50 mg/kg body weight of both commercial mixtures. The fishes were sacrificed at day 7 (n = 5 for each group) and 14 (n = 6 for each group), and blood, liver, fillet, and brain were collected. Blanks and positive controls were also part of the experiment. The expressions of Vtg, Zrp, and CYPIA were measured with several techniques (EROD, ELISA, Western, Northern and Slot Blot). The values in the groups of fish treated with Penta-BDE or Octa-BDE did not significantly differ from the reference group for any of the parameters tested. In contrast, the positive control groups treated with estradiol-17beta for Vtg and Zrp expression, and beta-naphthoflavone for CYP1A expression did show a significant response, indicating the potential sensitivity of the fishes for the parameters measured. Since the results of the chemical analyses showed concentrations of a number of PBDE congeners in liver, fillet, and brain that were about three orders of magnitude above those of fish from the North Sea, it is concluded that the short-term toxicity of both commercial PBDE mixtures for these endpoints was low.  相似文献   

An aromatase inhibitor and tamoxifen decrease plasma levels of o,p'-DDT and its metabolites in Nile tilapia (Oreochromis niloticus)     

Leaños-Castañeda O  Gold-Bouchot G  Van Der Kraak G  Lister A  Ceja-Moreno V  Simá-Alvarez R 《Marine environmental research》2004,58(2-5):337-342

The objective of this study was to determine if tamoxifen or an aromatase inhibitor (4-hydroxyandrostenedione; 4-OHA) affected plasma concentrations of o,p' -DDT and its metabolites, o,p'-DDD and o,p'-DDE, in mature tilapia. Male and female tilapia were injected 6 times intraperitoneally with o,p'-DDT (40 mg/kg) alone or in combination with 4-OHA (2 mg/kg) over a 12 day period. An additional group of male fish was injected with tamoxifen (5 mg/kg) plus o,p'-DDT. At the end of the treatment period, plasma samples were extracted and analyzed by GC/ECD. Females injected only with o,p'-DDT had significantly higher levels of o,p'-DDT compared with males. Interestingly, females and males treated concomitantly with o,p'-DDT and 4-OHA or tamoxifen had significantly lower concentrations of plasma o,p'-DDT (about 50%) compared with fish treated with only o,p'-DDT. These initial results suggest that an interaction between endocrine-active compounds occurs in vivo in tilapia and may involve alterations in metabolism of o,p'-DDT.  相似文献   

两株琼胶酶高产细菌的筛选和鉴定   总被引：4，自引：1，他引：4  

杜宗军  王鹏  李筠  P.A.W.Robertson  B.Austin 《海洋科学》2002,26(3):1-4

从海洋环境中筛选得到两株琼胶酶的高产菌株，通过形态观察和生理生化反应，确定它们属于弧菌属，通过BIOLOG细菌鉴定系统鉴定，并同弧菌属标准菌株分析比较，确定上方宝剑两株菌都是塔式弧菌（Vibrio tubiashii)，这两株菌在碳源利用方面存在差别。  相似文献   

Esterases as biomarkers in Nereis (Hediste) diversicolor exposed to temephos and Bacillus thuringiensis var. israelensis used for mosquito control in coastal wetlands of Morbihan (Brittany,France)     

Fourcy D  Jumel A  Heydorff M  Lagadic L 《Marine environmental research》2002,54(3-5):755-759

Since 1998, a biomonitoring programme has been implemented to assess the potential impact of chemical mosquito control on macroinvertebrates of the coastal wetlands of Morbihan (Brittany, France). Acetylcholinesterase and carboxylesterases were used as biomarkers to assess the effects of Abate 500e (a.i. temephos) and Vectobac 12 AS (a.i. endotoxins of Bacillus thuringiensis var. israelensis, Bti) in Nereis (Hediste) diversicolor. Esterase inhibition revealed a marked impact of temephos, suggesting preferential contamination of the worms through the food. In Bti-exposed N. diversicolor, random variations of esterase activities were observed, that could not be attributed to the larvicide. However, esterases only reflected indirect physiological effects of Bti, and further investigations are needed to identify biomarkers more specific of Bti endotoxins.  相似文献   

龙须菜在滩涂贝藻混养系统中的生态作用模拟研究   总被引：4，自引：0，他引：4  

孙伟  张涛  杨红生  刘保忠  周毅  刘鹰 《海洋科学》2006,30(12):72-76

2005年5-7月,在室内采用实验生态学方法对壳长为48.35 mm±4.27 mm文蛤(Meretrix meretrix Linnaeus)成贝和龙须菜(Gracilaria lemaneiformis Weber-van Bosse)的4种配比(生物量配比分别为0∶1、1∶1、2∶1、4∶1)进行了混养实验,每周测定养殖水体中营养盐变化情况以及文蛤、龙须菜的存活和生长情况等。实验表明,加入了大型藻类的文蛤养殖系统,相对于空白对照组,其氨氮、亚硝氮和磷酸盐浓度显著降低,NH4-N从5.52μmol/L降至1.30μmol/L、NO2-N从0.31μmol/L降至0.06μmol/L,而PO4-P从0.48μmol/L降至0.006μmol/L。实验结束后,文蛤及龙须菜生长情况良好,文蛤特定生长率最高达到了0.84%,龙须菜最高则达到了1.79%。本实验条件下,在文蛤成贝养殖系统中加入大型藻类,对养殖水体中的NH4-N、NO2-N、PO4-P有明显的吸收效果,吸收率分别为86%、98%和99%,起到了良好的生态作用。  相似文献   

刺参夏眠的研究进展   总被引：2，自引：2，他引：2  

袁秀堂  杨红生  陈慕雁  高菲 《海洋科学》2007,31(8):88-90

刺参(Apostichopus japonicus(Selenka)),又称仿刺参,属棘皮动物门(Echinodermata),海参纲(Ho-lothuroidea)[1,2],主要分布于北纬35°到44°的广大西北太平洋沿岸,北起俄罗斯的海参威,经日本海、朝鲜半岛南部到中国黄、渤海[3,4]。据报道,江苏连云港外的平山岛是该物种在中国分布的最南界[5]。刺参是典型的温带种类,在其生活史中存在着一种重要的生态习性:当夏季海水温度升高到一定范围后,刺参即迁移到海水较深、较安静的岩石间不动不食,这种现象称为“夏眠”(aestivation)。日本学者Mit-sukuri[6,7]最早观察到刺参的这一现象并提出了夏眠…  相似文献   

俄罗斯沿岸更新世盆地类型     

A A Cвиотч  朱佛宏 《海洋地质前沿》2004,20(10):30-31

俄罗斯沿岸地区受到分别属于大西洋、北冰洋和太平洋的14个海的海水冲刷，海岸线的总长度超过60000km。在更新世，这些盆地经历过不止一次的幅度不同的海平面的升降作用。在海退时期，广阔的陆架地区变干了，而在海侵时期，沿岸平原和低山岸区被海水淹没。在东北  相似文献   

Development of two novel CYP-antibodies and their use in a PCB exposure experiment with Mytilus edulis     

Jonsson H  Sandnes KV  Schiedek D  Schneider R  Grøsvik BE  Goksøyr A 《Marine environmental research》2004,58(2-5):655-658

In an attempt to learn more about the cytochrome P450 (CYP) system of mussels, we used protein databases and alignment software to extract highly conserved CYP sequences. From these alignments synthetic peptides were produced and used for rabbit immunisation, which yielded polyclonal antibodies against the CYP families 2 and 4. The antibodies were evaluated with Western Blot and ELISA assays, using digestive gland microsomal samples from the mussel Mytilus edulis. Western Blots revealed immunoreactions for both antibodies. The anti-CYP2 sequence rendered one major immunopositive protein of approximately 49 kDa size, and weak signals for proteins of approximately 41 and 56 kDa size. The anti-CYP4 sequence rendered two major bands of approximately 56 and 59 kDa size, and also a weak immunoreaction with a protein of approximately 43 kDa size. ELISA rendered only weak signals even with a 1:50 dilution of IgG-purified serum. A 10-day exposure to Aroclor 1254 did not appear to affect any of the immunopositive proteins, while total PCBs in soft bodies increased from 14-40 ng/g DW in controls to 373-638 ng/g DW in exposed mussels.  相似文献   

烟台海胆体内的纤毛虫和太平洋海胆体内纤毛虫的地理分布     

A.斯特列科夫 《海洋与湖沼》1959,2(1):61-67

1957年夏,中苏海洋动物综合考察队在黄海沿岸烟台地区工作时,参加考察队的阿·阿·斯特列科夫教授采集了海胆肠内的纤毛虫,并以此作为这篇文章的材料。在本文内将提出所见到的纤毛虫名称,并分析它们在太平洋地理分布的情况。  相似文献   

国外水下作业机械手的特点及发展趋势     

杨农林  茅及愚  向忠祥 《海洋科学》1990,14(6):67-69

国外水下作业机械手(以下简称机械手)的研究始于50年代。70年代以来,国外许多公司如美国的Schilling公司,Western Space and Marine有限公司以及英国的Slingsby有限公司等,相继研制成功了多种先进的机械手及系列产品,并已投入批量生产,使机械手的研究和生产达到了较高的水平。我国水下作业机械手的研制工作始于70年代,与国外相比还存在着一段差距。本文从水下机械手的结构、液压驱动及控制  相似文献